B.S., North Carolina State University (2000)
Ph.D., Princeton University (2006)

My research program utilizes bioanalytical mass spectrometry and organic synthesis to both develop analytical tools and to probe biological phenomena, with a specific focus on understanding signaling systems and their links to metabolism. My lab has established collaborations with a number of other researchers, both at UTK and at other universities. By utilizing interdisciplinary environments both within my lab and among collaborators, the students in my lab are able to experience differing scientific perspectives. The organic group in the Campagna lab focuses on the synthesis of biologically relevant compounds, The largest of these initiatives is an ongoing collaboration with Dr. Jason Collier at Pennington Biomedical Research Center, aiming to design and synthesize novel glucocorticoid analogues that maintain their anti-inflammatory properties while minimizing their potential to induce unwanted side effects, such as steroid induced diabetes. This project is further enhanced with computational experiments, including Molecular Dynamic simulations and de novo drug design to better understand the structure-activity relationship between these ligands and their target protein, the glucocorticoid receptor. We have also started an exciting and highly collaborative medicinal chemistry project to develop novel radioconjugates for PET imaging and targeted cancer therapy. While the analytical group is involved in a wide array of collaborations, one of the main focuses has been to understand the small molecule produced by the gut microbiome as these regulate our overall health. The small molecule profile produced by the microorganisms that inhabit our bodies is highly individualized and sensitive to intrinsic (age, sex, ethnicity) and extrinsic factors (diet, geography, lifestyle), making it an ideal target in precision medicine. In addition, our lab is investigating the mechanisms behind quorum sensing, a cell-to-cell communication process that bacteria use to coordinate their behaviors. These multidisciplinary projects require the synthesis of novel metabolites and the analysis of mixture of molecules to understand how bacterial metabolism is regulated by these fascinating organisms.

Atlantic Richfield Professor 2012-2015

Burke, S. J., May, A. L., Noland, R. C., Lu, D., Brissova, M., Powers, A. C., Sherrill, E. M., Karlstad, M. D., Campagna, S. R., Stephens, J. M., Collier, J. J., “Thiobenzothiazole-modified hydrocortisones display anti-inflammatory activity with reduced impact on islet β-cell function.” Journal of Biological Chemistry, 2015, 290(21), 13401-13416.

Im, J., Prevatte, C.W.,* Campagna, S. R., Löffler, F. E., “Identification of 4-hydroxycumyl alcohol as the major MnO₂-mediated bisphenol A transformation product and evaluation of its environmental fate.” Environmental Science and Technology, 2015, 49(10), 6214-6221.

Dunham, B. P., Sharma, S., Luo, H., Smith, C. B., Amin, S. A., Bender, S. J., Dearth, S. P., * Van Mooy, B. A. S., Cmpagna, S. R., Kujawinski, E. B., Armbrust, E. V., Moran, M. A., “Cryptic carbon and sulfur cycling between surface ocean plankton.” PNAS USA, 2015, 112(2), 453-457.

Cheema, M., Mohan, M. S., Campagna, S. R., Jurat-Fuentes, J. L., Harte, F. M., “The association of low-molecular-weight hydrophobic compounds with native casein micelles in bovine milk.” Journal of Dairy Science, 2015, 98(8), 5155-5163.

Rymer, J. K., Hauser, M., Bourdon, A. K.,* Campagna, S. R., Naider, F., Becker, J. M., “Novobiocin and peptide analogs of a-factor are positive allosteric modulators of the yeast G protein-coupled receptor Ste2p.” Biochimica et Biophysica Acta – Biomembranes, 2015, 1848(4), 916-924.

Cude, W. N., Prevatte, C. W.,* Hadden, M. K., Smith, R. T.,** Swain, C. L.,** Campagna, S. R., Buchan, A. “Phaeobacter sp. strain Y4I utilized two separate cell-to-cell communication systems to regulate production of the antimicrobial indigoidine.” Applied and Environmental Microbiology, 2015, 81(4), 1417-1425.

Richardson, L. L., Stanic, D., May, A. L.,* Brownell, A., Gantar, M., Campagna, S. R., “Ecology and physiology of the pathogenic cyanobacterium Roseofilum reptotaenium.” Life, 2014, 4(4), 968-987.

Zimmer, B. L., May, A. L.,* Bhedi, C.D., Dearth, S. P.*, Prevatte, C. W.,* Pratte, Z., Campagna, S. R., Richardson, L. L., “Quorum sensing signal production and microbial interactions in a polymicrobial disease of corals and the coral surface mucopolysaccharide layer.” PLoS One, 2014, 9(9), e108541.

Steffen, M. M.,* Dearth, S. P.,* Dill, B. D., Li, Z., Campagna, S. R., Wilhelm, S. W., “Nutrients drive transcriptional changes that maintain metabolic homeostatsis but alter genome architecture in Microcystis.” ISME Journal, 2014, 8(10), 2080-2092.

Steill, J. D., May, A. L.,* Campagna, S. R., Oomens, J., Compton, R. N. “Structure and Stability of Phenoxide and Fluorophenoxide Anions Investigated with Infrared Multiple-Photon Dissociation and Detachment Spectroscopy and Tandem Mass Spectrometry.” Journal of Physical Chemistry A, 2014, 118(37), 8597-8605.

Artegoitia, V. M.,* Middleton, J. L.,* Harte, F. M., Campagna, S. R., de Veth, M. J., “Choline and Choline Metabolite Patterns and Associations in Blood and Milk during Lactation in Dairy Cows.” PLoS One, 2014, 9(8), e103412.

Im, J., Prevatte, C. W.,* Lee, H. G., Campagna, S. R., Löffler, F. E., “4-methylphenol produced in freshwater sediment microcosms is not a bisphenol A metabolite.” Chemosphere, 2014, 117(1), 521-526.

Ji, B,* Middleton, J. L.,* Ernest, B.,* Saxton, A. M., Lamont, S. J., Campagna, S. R., Voy, B. H. “Molecular and metabolic profiles suggest that increased lipid catabolism in adipose tissue contributes to leanness in domestic chickens.” Physiological Genomics, 2014, 46(9), 315-327.

Kolker, E., campagna, S. R., dearth, S. P.,* May, A. L.,* et al. “Toward More Transparent and Reproducible Omics Studies Through Common Metadata Checklist and Data Publications.” Omics: A Journal of Integrative Biology/Big Data, 2014, 18(1), 10-14.

Ankrah, N.,* May, A. L.,* Middleton, J. L.,* Jones, D.,** Hadden, M., Gooding, J. R.,* LeCleir, G., Wilhelm, S. W., Campagna, S. R. “Phage infection of an environmentally relevant marine bacterium alters host metabolism and lysate composition” ISEM J, 2013, 8(5), 1089-1100.

Kosentka, P.,* Sprague, S. L.,** Ryberg, M., Gartz, J., May, A. L.,* Campagna, S. R., Matheny, P. B. “Evolution of the Toxins Muscarine and Psilocybin in a Family of Mushroom-Forming Fungi.” PLoS One, 2013, 8(5), e64646.

Russell, M. H.,* Bible, A. N.,* Fang, X., Gooding, J. R.,* Campagna, S. R., Gomelsky, M., Alexandre, G., “Integration of the second messenger c-di-GMP into the chemotactic signaling pathway.” mBio, 2013, 4(2)

Ernest, J. B.,* Gooding, J. R.,* Campagna, S. R., Saxton, A. M., Voy, B. H., “MetabR: An R script for linear model analysis of quantitative metabolomic data.” BMC Research Notes, 2012, 5:596.

Ji, B.,* Ernest, B.,* Gooding, J. R.,* Das, S., Saxton, A. M., Dupont, J., Métayer-Coustard, S., Campagna, S. R., Voy, B. H., “Transcriptomic and metabolomic profiling of chicken adipose tissue in response to insulin neutralization and fasting” BMC Genomics, 2012

Saenz, G. C., Underwood, A., Belapure, S. A.,* Campagna, S. R., Sun, Z., Tammariello, S., Rickard, A. H.  “Autoinducer-2 influences interactions amongst pioneer colonizing Streptococci in oral biofilms.” Microbiology, 2012, 158(7), 178301795.

Tonner, R., Schwerdtfeger, P., May, A. L.,* Steill, J. D., Bergen, G., Oomens, J., Campagna, S. R., Compton, R. N. “Stability of gas-phase tartaric acid anions investigated by quantum chemistry, mass spectrometry, and infrared spectroscopy.” Journal of Physical Chemistry A, 2012, 116(19), 4789-4800.

May, A. L.,* Eisenhauer, M. E.,* Coulston, K. S.,** Campagna, S. R. “Detection and quantitation of bacterial acylhomoserine lactone quorum sensing molecules via liquid chromatography–isotope dilution tandem mass spectrometry.” Analytical Chemistry, 2012, 84(3), 1243-1252.

Van Mooy, B. A. S., Hmelo, L. R., Mincer, T. J., Sofen, L. E., Campagna, S. R., May, A. L.,* Dyhrman, S. T., Webb, E. A., Momper, L. “Quorum sensing control of phosphorus acquisition in Trichodesmium colonies.” The ISME Journal, 2012, 48, 422-429.

Van Mooy, B. A. S.; Hmelo, L. R.; Mincer, T. J.; Sofen, L. E.; Campagna, S. R.; May, A. L.; Dyhrman, S. T.; Webb, E. A.; Momper, L.,  Quorum sensing control of phosphorus acquisition in Trichodesmium colonies. The ISME Journal, 8 September 2011; doi:10.1038/ismej.2011.115

Clasquin, M. F.; Melamud, E.; Singer, A.; Gooding, J. R.; Xu, X.; Dong, A.; Cui, H.; Campagna, S. R.; Savchenko, A.; Yakunin, A. F.; Rabinowitz, J. D.; Caudy, A. A., Riboneogensis in yeast. Cell, 2011, 145(6), 969-980.

Collier, J.J.; Burke, S. J.; Eisenhauer, M. E.; Lu, D.; Sapp, R. C.; Frydman, C. J.; Campagna, S. R., Pancreatic b-cell Death in Response to Pro-inflammatory Cytokines is Distinct from Genuine Apoptosis. PLoS One, 2011, 6(7), e22485.

Gooding, J. R.; May, A. L.; Hilliard, K. R.; Campagna, S. R., Establishing a Quantitative Definition of Quorum Sensing Provides Insight into the Information Content of the Autoinducer Signals in Vibrio harveyi and Escherichia coli. Biochemistry, 2010, 49(27), 5621-5623.

Fairchild J. N.; Horvath, K.; Gooding, J. R.; Campagna, S. R.; Guiochon, G., Two-dimensional liquid chromatography/mass spectrometry/mass spectrometry separation of water-soluble metabolites. J. Chromatography A,2010, 1217(52), 8161-6.

Campagna, S. R.; Gooding, J. R.; May, A. L., Direct Quantitation of the Quorum Sensing Signal, Autoinducer-2, in Clinically Relevant Samples by Liquid Chromatography-Tandem Mass Spectrometry. Analytical Chemistry 2009, 81 (15), 6374-6381.

Semmelhack, M. F.; Campagna, S. R.; Federle, M. J.; Bassler, B. L. “An expeditious synthesis of DPD and boron binding studies,” Org. Lett., 2005, 7(4), 569-572.

Miller, S. T.; Xavier, K. B,; Campagna, S. R.; Taga, M. E.; Semmelhack, M. F.; Bassler, B. L.; Hughson, F. M. “Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal Al-2,” Mol. Cell, 2004, 15(5), 677-687.