The Calhoun Lab was published in the Journal of Physical Chemistry C for their research “Leaving the Limits of Linearity for Light Microscopy” and it is a perspective on the recent advances in the field of nonlinear microscopy.
Graduate student authors include Marea Blake and Brandon Colon. Blake is currently pursuing her Ph.D. in Chemistry at the University of Tennessee, Knoxville. Her current research focuses on probing small molecule-membrane interactions in living cells using nonlinear techniques such as second harmonic generation and two-photon fluorescence.
Colon received his B.S. degree (2016) in Chemistry from the University of West Florida in Pensacola, FL. He is a doctoral student at the University of Tennessee under the tutelage of Prof. Tessa Calhoun. He has been investigating the use of total internal reflection illumination geometries to apply nonlinear microscopy techniques to microfluidic samples.
In this paper, the group highlights recent developments within the past couple of years pertaining to how nonlinear microscopy methods such as transient absorption, 2D nonlinear microscopy, second order processes, and quantum microscopy are being implemented to probe different timescales, access information on interfaces and illuminate samples with novel excitation schemes.
“Our group actually uses a few of these methods (such as TAM, SHG and TIR geometry excitation) in our lab so it was really exciting getting to portray that in this perspective and explore the directions they can grow,” Blake said.