• Request Info
  • Visit
  • Apply
  • Give
  • Request Info
  • Visit
  • Apply
  • Give

Search

  • A-Z Index
  • Map

Chemistry

  • About
    • Student Organizations
    • Connect With Us
    • Careers With Us
    • Employee/Student Travel Request
    • Share Your Dr. Schweitzer Story
  • Undergraduate Students
    • Majors and Minors
    • First Year Students
    • Undergraduate Research
    • Summer Programs
    • Chemistry Lab Excused Absence
    • Apply
  • Graduate Students
    • Our Programs
    • Graduate Student Resources
    • Research Open House
    • Apply
  • Faculty
  • People
  • Research
    • Research Areas
    • Facilities
  • News
Home » Heberle

Heberle

Heberle Lab Published in Data in Brief

May 25, 2021 by Kayla Benson

Heberle Lab Published their research “Dataset of asymmetric giant unilamellar vesicles prepared via hemifusion: Observation of anti-alignment of domains and modulated phases in asymmetric bilayers” in Data in Brief.

The data provided with this paper are confocal fluorescence images of symmetric giant unilamellar vesicles (GUVs) and asymmetric giant unilamellar vesicles (aGUVs). In this work, aGUVs were prepared using the hemifusion method and are labelled with two different fluorescent dyes, named TFPC and DiD. Both dyes show strong preference for the liquid-disordered (Ld) phase instead of the liquid-ordered (Lo) phase. The partition of these dyes favoring the Ld phase leads to bright Ld phase and dark Lo phase domains in symmetric GUVs observed by fluorescence microscopy. In symmetric vesicles, the bright and the dark domains of the inner and the outer leaflets are aligned. In aGUVs, the fluorescent probe TFPC exclusively labels the aGUV outer leaflet.

Here, they show a dataset of fluorescence micrographs obtained using scanning fluorescence confocal microscopy. For the system chosen, the fluorescence signal of TFPC and DiD show anti-alignment of the brighter domains on aGUVs. Important for this dataset, TFPC and DiD have fluorescence emission centered in the green and far-red region of the visible spectra, respectively, and the dyes’ fluorescence emission bands do not overlap. This dataset were collected in the same conditions of the dataset reported in the co-submitted work (Enoki, et al. 2021) where most of aGUVs show domains alignment. In addition, they show micrographs of GUVs displaying modulated phases and macrodomains. They also compare the modulated phases observed in GUVs and aGUVs. For these datasets, they collected a sequence of micrographs using confocal microscopy varying the z-position, termed a z-stack. Images were collected in a scanning microscope Nikon Eclipse C2+ (Nikon Instruments, Melville, NY). Additional samples used to measure the lipid concentrations and to prepare GUVs with accurate lipid fractions are also provided with this paper.

Filed Under: Heberle

Heberle Published in BBA – Biomembranes

May 2, 2021 by Kayla Benson

The Heberle Lab published their research “Investigation of the domain line tension in asymmetric vesicles prepared via hemifusion” Biochimica et Biophysica Acta Biomembranes.

The plasma membrane (PM) is asymmetric in lipid composition. The distinct and characteristic lipid compositions of the exoplasmic and cytoplasmic leaflets lead to different lipid-lipid interactions and physical-chemical properties in each leaflet. The exoplasmic leaflet possesses an intrinsic ability to form coexisting ordered and disordered fluid domains, whereas the cytoplasmic leaflet seems to form a single fluid phase.

To better understand the interleaflet interactions that influence domains, the lab compared asymmetric model membranes that capture salient properties of the PM with simpler symmetric membranes. Using asymmetric giant unilamellar vesicles (aGUVs) prepared by hemifusion with a supported lipid bilayer, they investigate the domain line tension that characterizes the behavior of coexisting ordered + disordered domains. The line tension can be related to the contact perimeter of the different phases. Compared to macroscopic phase separation, the appearance of modulated phases was found to be a robust indicator of a decrease in domain line tension. Symmetric GUVs of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)/1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC)/cholesterol (chol) were formed into aGUVs by replacing the GUV outer leaflet with DOPC/chol = 0.8/0.2 in order to create a cytoplasmic leaflet model. These aGUVs revealed lower line tension for the ordered + disordered domains of the exoplasmic model leaflet.

Filed Under: Heberle

Recent Posts

  • 2025 Honors Day
  • 2025 Undergraduate Awards
  • Baccile Awarded $1.8 Million Grant for Pioneering Research on Five-Carbon Metabolism
  • UT Chemistry Lab Explores Dipeptides for Carbon Dioxide Capture
  • Chemical Bonds – Fall 2024

Recent Comments

No comments to show.

College of Arts & Sciences

117 Natalie L. Haslam Music Center
1741 Volunteer Blvd.
Knoxville TN 37996-2600

Phone: 865-974-3241

Archives

  • May 2025
  • April 2025
  • March 2025
  • December 2024
  • November 2024
  • October 2024
  • September 2024
  • August 2024
  • July 2024
  • June 2024
  • May 2024
  • April 2024
  • March 2024
  • February 2024
  • December 2023
  • November 2023
  • September 2023
  • July 2023
  • June 2023
  • May 2023
  • April 2023
  • March 2023
  • January 2023
  • December 2022
  • November 2022
  • July 2022
  • June 2022
  • May 2022
  • April 2022
  • March 2022
  • February 2022
  • January 2022
  • December 2021
  • November 2021
  • October 2021
  • July 2021
  • June 2021
  • May 2021
  • April 2021
  • March 2021
  • February 2021
  • January 2021
  • December 2020
  • November 2020
  • October 2020
  • September 2020
  • August 2020
  • July 2020
  • June 2020
  • May 2020
  • April 2020
  • March 2020
  • February 2020
  • January 2020
  • November 2019
  • October 2019
  • September 2019
  • August 2019
  • July 2019
  • May 2019
  • April 2019
  • March 2019
  • February 2019
  • September 2018
  • July 2018
  • June 2018
  • December 2017
  • October 2017
  • September 2017
  • August 2017
  • July 2017
  • June 2017
  • May 2017
  • April 2017
  • March 2017
  • January 2017
  • December 2016
  • November 2016
  • October 2016
  • September 2016
  • August 2016
  • June 2016
  • May 2016
  • April 2016
  • February 2016
  • January 2016
  • December 2015
  • November 2015
  • October 2015
  • August 2015
  • July 2015
  • June 2015
  • May 2015
  • April 2015
  • March 2015
  • February 2015
  • November 2014
  • October 2014
  • September 2014
  • August 2014
  • July 2014
  • June 2014
  • May 2014
  • April 2014
  • March 2014
  • February 2014
  • January 2014
  • December 2013
  • November 2013
  • October 2013
  • September 2013
  • August 2013
  • July 2013
  • June 2013
  • May 2013
  • April 2013
  • March 2013
  • February 2013
  • January 2013
  • September 2012
  • August 2012
  • July 2012
  • June 2012
  • May 2012
  • April 2012
  • February 2012
  • January 2012
  • December 2011
  • October 2011
  • August 2011
  • July 2011
  • June 2011
  • May 2011
  • April 2011
  • March 2011
  • January 2011
  • November 2010
  • October 2010
  • September 2010
  • August 2010
  • July 2010
  • June 2010

Categories

  • ACGS
  • alumni
  • Analytical Chemistry
  • Artsci
  • award
  • Bailey
  • Best
  • BOV
  • Brantley
  • Calhoun
  • Campagna
  • Dadmun
  • Dai
  • Darko
  • Do
  • endowment
  • faculty
  • Faculty
  • Featured
  • fellowship
  • Graduate Student Spotlight
  • Graduate Students
  • Hazari
  • Heberle
  • Inorganic Chemistry
  • Jenkins
  • Kilbey
  • Larese
  • Long
  • Musfeldt
  • NCW
  • Nemykin
  • News
  • newsletter
  • Organic Chemistry
  • Physical Chemistry
  • Polymer Chemistry
  • Sharma
  • Sokolov
  • Uncategorized
  • undergraduate
  • Undergraduate Student Spotlight
  • Vogiatzis
  • Xue
  • Zhao

Copyright © 2025 · University of Tennessee, Knoxville WDS Genesis Child on Genesis Framework · WordPress · Log in

Chemistry

College of Arts & Sciences

552 Buehler Hall
1420 Circle Dr.
Knoxville, TN 37996-1600

Email: chemistry@utk.edu

Phone: 865-974-3141

 

The University of Tennessee, Knoxville
Knoxville, Tennessee 37996
865-974-1000

The flagship campus of the University of Tennessee System and partner in the Tennessee Transfer Pathway.

ADA Privacy Safety Title IX